Sharp PC-9300 Informacje Techniczne Pobierz pdf (Strona 32)

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4 Absorbance

4.1 General Information

• Four installed filters and two customizable filter holders are included on the six-position filter

wheel.

• The absorbance LED spectral range is 400 to 800nm.

• Microplate recommendations:

- Black wall and clear flat bottom are recommended.

- Clear microplate can be used.

• Two absorbance wavelengths can be selected per well for running ratiometric assays.

4.2 Optical Filters Wheel

The optical filter wheel located on the bottom of the absorbance detection head holds four fixed

optical filters and two customizable filter paddles. The wavelength is easily selected using the

touch screen interface and the filter is selected automatically by the instrument. A manual change

of the filter is not required.

Filter Wavelength

(nm)

Assays

450

ELISA, QuantiCleave Protease Assay

560

BCA Protein Assays

600

Bradford Protein Assays, Coomasssie Blue

Protein Assays, PeroXOquant Quantitative

Peroxide Assay

750

Lowry Protein Assays

Table 4-1: Optical Wavelengths for Various Assays

1 2 ... 27 28 29 30 31 32 33 34 35 36 37 ... 67 68

Brak uwag

Sharp PC-9300 Informacje Techniczne Strona 32